WebThe in vitro unscheduled DNA synthesis assay (UDS) is part of the routine genetic toxicology screening at The Upjohn Company. The purpose of this paper is to report results for 8 … Web29 Mar 2011 · Human hepatocytes were isolated from explanted diseased livers from patients with alcoholic liver disease (ALD), biliary cirrhosis (primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC) and a variety of other end-stage liver diseases (Cystic Fibrosis, Cryptogenic Fibrosis, Alpha-1-antitypsin Deficiency, Autoimmune …

Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver …

WebPrimary cultures of adult rat hepatocytes were incubated for 18-20 h with 6 concentrations (0.1, 1, 10, 100, 1,000, 10,000 μg/ml). Benzo (a)pyrene was also tested and served as the positive controls. Unscheduled DNA synthesis was measured by autoradiography. Web3.2 Primary Culture of Hepatocytes 1. The liver is transferred to a sterile, plastic Petri dish, cut open, and the hepatocytes are carefully teased out using a spatula. 2. All Williams-E media should be prewarmed to 37°C. 3. The resulting hepatocyte suspension is gently filtered through 150 μm nylon bolting cloth into a glass beaker. massachusetts section 12

Rat Primary Hepatocytes - Plateable - Cell Biologics

WebThe in vitro unscheduled DNA synthesis (UDS) assay in rat primary hepatocytes: evaluation of 2-furoic acid and 7 drug candidates. (English) 1 reference. stated in. Europe PubMed … Web31 Jan 2012 · All 32 rats were examined for the presence of lung tumours; in 26 of the 32 the possibility of liver tumours was investigated as well (!). In the exposed group lung tumours were found in 25 % of the animals and liver … WebStatistical evaluation of the number of cells scored in the rat primary hepatocyte unscheduled dna synthesis uds assay ... Wiser, S.K.; Grzegorczyk, C.R.; Smith, A.L. 1989: … massachusetts section 12e