Raw-blue cells
WebRAW-Blue cells were treated with the shown concentrations of FP20 and incubated for 16–18 h. MPLA and LPS (100 ng/mL) were used as controls. The 100% stimulation has been assigned to the positive control LPS. Data are expressed as mean ± SEM of at least three independent experiments ... WebRAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. InvivoGen has developed RAW-Blue ™ cells and RAW-Lucia ™ cells, which express a secreted inducible ...
Raw-blue cells
Did you know?
WebRAW‑Dual™ cells stably express two reporter genes encoding SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase . SEAP expression depends on the activation of the endogenous MIP-2 promoter. MIP‑2, also known as CXCL2, is the murine homolog of human IL-8, a chemokine produced in an NF-κB-dependent manner [3]. WebApr 22, 2015 · S2 Fig. RAW-BLUE cells detect trace levels of endotoxin in heparin lots. RAW-BLUE cells were cultured with 10 different lots of heparin (#1–10) in the presence or absence of 100pg/mL endotoxin for 24h. TNF-α levels were measured by ELISA in cell supernatant at 24 h. Each point represents mean ± SD of triplicate cell culture.
WebFeb 17, 2024 · Coculture D1 Cells:RAW-Blue Cells Additionally, we prepared cocultures of D1 and RAW-blue cells using transwell inserts without the Dex P-A hydrogel. After 24 h incubation at 37 °C, NF-κB/AP-1 activation upon the previously mentioned stimuli was assessed by measuring the release of secreted embryonic alkaline phosphates (SEAPs) … WebCo-immunoprecipitation experiments using cell lysates from RAW-blue cells further dem- onstrated that Neu1 forms a complex with TLR9 receptors in naive and ODN treatment of cells for 30 min (Fig. 4C).
WebJun 29, 2024 · RAW-Blue macrophages were purchased from InvivoGen (San Diego, CA). All cells were propagated in RPMI 1640 supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 °C in a 5% CO 2 incubator [16, 17]. Cell viability assay. Cell death was quantified by the MTT reduction assay. Cell viability was quantified by the Alamar …
WebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions.
WebRAW 264.7. TIB-71 ™. RAW 264.7 is a macrophage cell line that was established from a tumor in a male mouse induced with the Abelson murine leukemia virus. Product category. can gerd last for yearsWebJun 30, 2011 · During the course of blocking CD36 oxLDL binding function using anti CD36 antibodies, we have identified a novel domain of CD36 that triggers inflammatory response-independent of oxLDL binding. OxLDL bound to the mouse reporter cell line RAW-Blue induced TNF-α and RANTES mRNA and protein expression. Pretreatment of RAW-Blue … can gerd lead to gastritisWebSep 6, 2024 · RAW-Blue and THP-1-Blue cells were plated at 1x10 6 cells/ml in flat bottom 96-well plates in a 100 µL volume. Excipients and TLR ligands were added for a final volume of 200 μL. After 24 h of stimulation, supernatants were collected and NF-κB activation was measured using the QUANTI-Blue detection medium according to manufacturer … can gerd lead to heart failureWebRAW-Blue™ ISG cells allow the detection of bioactive murine type I IFNs or PAMPs by monitoring the activation of the interferon regulatory factor (IRF) pathway. They are derived from the RAW 264.7 macrophages. RAW-Blue™ ISG cells stably express a secreted embryonic alkaline phosphatase (SEAP) gene under the control of the interferon-stimulated can gerd lead to cancerWebSep 30, 2024 · Supernatant from the treated cells was collected and SEAP production was detected over a period of 45 minutes at 655 nm using the QUANTI-Blue ™ detection medium. Detection of protein expression by immunoblotting and densitometry. Cells from the treated well-plate were lysed and saved in the -80 ° C freezer for can gerd irritate the vagus nerveWebResults: Alamar blue cell viability assay showed no toxicity to RAW cells with the use of velutin in all concentrations tested (p>0.05). Velutin did not induce cell apoptosis based on caspase 3/7 assay (p>0.05). Fluorescence images stained by DAPI showed no alteration in the morphology of RAW cell nuclei (p>0.05) treated with velutin. can gerd lead to throat cancerWebJan 16, 2024 · Then you can follow this protocol: 1- Plate 1–2 * 10^4 adherent cells or 1–2 * 10^4 to 1 * 10^5 suspension cells in 100μl per well of a 96-well plate. Incubate overnight. 2- Allow the TR to ... fitbit users by country