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Illumina HiSeq2500, HiSeq4000, and NovaSeq - Yale Center for Genome
WebSequencing on HighSeq2500 Reference sequence Paired end reads Seq primer Reads 100 bp Flip Seq primer Reads 100 bp Paired-End Reading (2X100 bp) • Increase read coverage … WebJul 17, 2014 · The resulting cDNAs were separated on a 4% agarose E-Gel (Invitrogen, Life Technologies), and the 140–160 nt length species were selected. cDNA was purified using the MinElute Gel Extraction kit (QIAGEN). Libraries were sequenced using an Illumina HighSeq2500 instrument. mRNA Library Preparation and Sequencing cure gallstones at home
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